Cytochemical Staining of the Golgi Apparatus in Amoeba Proteus
نویسندگان
چکیده
The Golgi apparatus has been shown to be involved in the packaging of secretory proteins (Caro and Palade, 1964 ; Jamieson and Palace 1967 a, 1967 b), and in the production of acid mucopolysaccharides and glycoproteins (Neutra and Leblond, 1966 ; Berlin, 1967 ; Thiéry, 1967 ; Rambourg et al, 1969; Whur et al., 1969) . Other suggested functions of the Golgi apparatus include the formation of the plasma membrane
منابع مشابه
The appearance of carbohydrate-rich material in the developing Golgi apparatus of amoebae.
The silver proteinate reaction was used to stain carbohydrate-rich substances in normal Amoeba proteus and in the developing Golgi apparatus of renucleated amoebae. Normal cells contained stained material, which probably is glycoprotein, in the cell surface, cisternae at the concave pole of the Golgi apparatus, and cytoplasmic vesicles and vacuoles. Previous radioautographic studies had shown t...
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The appearance of enzymic activity during the development of the Golgi apparatus was studied by cytochemical staining of renucleated amoebae. In cells enucleated for 4 days, there was a great decline in size and number of Golgi bodies, or dictyosomes. Subsequent renucleation by nuclear transplantation resulted in a regeneration of Golgi bodies. Samples of amoebae were fixed and incubated for cy...
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To investigate the origin and nature of vesicles found within multivesicular bodies (mvb), the cytochemical staining properties of mvb vesicles were compared with those of other cytoplasmic vesicles, i.e. those associated with the Golgi complex and endocytic vesicles found near the apical cell surface. Rat epididymal tissue was stained in unbuffered OsO(4) for 40-48 hr, and the distribution of ...
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ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 46 شماره
صفحات -
تاریخ انتشار 1970